Figure 6.

GMP-AMP synthase (cGAS) and stimulator of interferon genes (STING) regulate the gene expression of interferon signalling-related genes in preadipocytes. (A) Western blot assessing the protein levels of cGAS and STING in control Non Targeting (NT), cGAS Knockout (KO), STING KO cells. Actin was used as loading control. (B) Gene expression analysis for interferon signalling-related genes (interferon β (IFNβ), interferon-stimulated gene 15 (ISG15), MX dynamin-like GTPase 2 (MX2), oligoadenylate synthetase-like 2 (OASL2) and C-X-C motif chemokine ligand 10 (CXCL10)) in untreated NT, cGAS KO, and STING KO preadipocytes vs. H₂O₂ treated counterparts as indicated. Results (Relative expression) are presented as means ±SEM from three independent experiments. ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001 (ANOVA with post hoc Tukey test).