Figure 8.

STAT1 functionally interacts with cGAS/STING to drive the expression of CXCL10 and antiviral response genes and STAT3 negatively regulates this interaction. (A) Western blot assessing the protein levels of cGAS, STING, IFNα, IFNβ, CXCL10 and IL6 in untreated NT, STAT1 KO, and STAT3 KO preadipocytes vs. H₂O₂ treated counterparts as indicated. Actin was used as loading control. Results are represented as means ± SEM from three independent experiments. (B) Gene expression analysis of most significantly upregulated SASP molecules in untreated NT, STAT1 KO, and STAT3 KO preadipocytes vs. H₂O₂ treated counterparts as indicated including IGFBP4, CP, and C3 along with interferon signalling-related genes (ISG15, MX2 and OASL2). Results (Relative expression) are presented as means ± SEM from three independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001 (ANOVA with post hoc Tukey test).