Figure 3.

Leaf senescence regulated by OsAAP3 is related to chloroplast degradation and cell death. Paraffin sections were used to detect the chloroplasts in the wild-type (WT) (A), overexpressing (OE) lines (B–D), and RNA interference (Ri) lines (E–G). Transmission electron sections were used to detect the chloroplasts in the WT (H,K), OE lines (I,L), and Ri lines (J,M). Paraffin sections (A–G) were stained with hematoxylin for 5 min and eosin for 2 min.The red frames in (H–J) indicate chloroplasts, the yellow arrows in (K–M) indicate thylakoids, and the red arrows in (K–M) indicate plastoglobules. The flag leaves was taken at 110 days after sowing. Scale bar = 30.0 mm in (A–G), 2.0 μm in (H–J), and 0.5 μm in (K–M).