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. 2020 Nov 15;31(24):2718–2732. doi: 10.1091/mbc.E20-06-0366

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© 2020 Pollitt et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 3: — The LIM domain and nebulin repeats are essential for LASP1 localization to the leading edge. (A) Live CAD cells coexpressing Lifeact-mRuby (F-actin, cyan) and wild-type GFP-LASP1, GFP-LASP1ΔLIM, GFP-LASP1ΔNebulin (GFP-LASP1ΔNeb), or GFP-LASP1ΔSH3 (magenta). Wild-type GFP-LASP1 and GFP-LASP1ΔSH3 localize to the leading edge. However, GFP-LASP1ΔLIM and GFP-LASP1ΔNeb do not localize to the leading edge and instead appear mostly soluble. Scale bars: 10 µm. (B) Line scan analysis of GFP-LASP1 localization at the leading edge (120 line scans from 30 cells across three independent culture replicates). Error bars represent standard error.