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. 2020 Nov 15;31(24):2687–2702. doi: 10.1091/mbc.E20-03-0200

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© 2020 Le et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 6: — OCA2 overexpression compensates for loss of SLC45A2-dependent melanosome neutralization in melanocyte pigmentation. SLC45A2-deficient melan-uw cells or OCA2-deficient melan-p1 cells were transiently transfected with negative CTRL HA-SLC35D3 (a, d), HA-SLC45A2 (b, e), or HA-OCA2 (c, f), fixed and labeled for HA (not shown). BF was used to visualize pigmented melanosomes within HA-positive cells. Scale, 10 μm. (g, h) The number of HA-containing pigmented cells was quantified and is depicted as a mean percentage of total HA-containing (HA+) cells ± SEM. Quantification is from the following independent experiments and total sample sizes: melan-uw + HA-SLC35D3 (3, 67), melan-uw + HA-SLC45A2 (9, 320), melan-uw + HA-OCA2 (9, 308), melan-p1 + HA-SLC35D3 (3, 62), melan-p1 + HA-SLC45A2 (4, 137), and melan-p1 + HA-OCA2 (4, 90). Statistical significance was determined using one-way ANOVA with Holm-Sidak’s test for multiple comparisons. *p < 0.05; ***p < 0.001; ****p < 0.0001; ns, not significant.