Fig. 2.

LncRNA ROR silencing inhibits proliferation and invasion, and promotes apoptosis of breast cancer cells. MCF-7 cells were treated with sh-lncRNA ROR or oe-lncRNA ROR. a Cell proliferation of MCF-7 detected by EdU. b Cell apoptosis and cycle of MCF-7 measured by flow cytometry. c Cell invasion of MCF-7 assessed by Transwell assay. d Protein levels of Vimentin, N-cadherin, MMP-2, MMP-9, Ki67, PCNA, Bcl-2, E-cadherin, Bax and Cleaved caspase-3 in MCF-7 determined by Western blot analysis. *p < 0.05 vs. MCF-7 cells treated with sh-NC. ^#p < 0.05 vs. MCF-7 cells treated with oe-NC. The above data are measurement data and expressed as mean ± standard deviation. Comparisons among multiple groups are analyzed by independent sample t test. The experiment is repeated 3 times. EdU, 5-Ethynyl-2′-deoxyuridine; MMP, matrix metalloproteinase; PCNA, proliferating cell nuclear antigen; Bax, Bcl-2-associated protein x; Bcl-2, B cell lymphoma 2; NC negative control