Fig. 3.

Panaxydol (PX) inhibits LPS-induced ALI and ferroptosis in vivo. Fe-citrate (III) (Fe) is dissolved in stroke-physiological saline solution (SPSS). PX is dissolved in dimethyl sulfoxide (DMSO), and further diluted in SPSS. Intravenous injection of Fe or/and intraperitoneal injection of PX are performed from day 0 to day 2. At 1 h after the final Fe and PX treatment, the mice are anesthetized with 30 mg/kg of pentobarbital sodium, and then LPS (10 µg/mouse) or SPSS is injected into the trachea. Then BALF and lung tissue are collected used as the following detections. a The lung histological changes are assessed by H&E staining. b The lung injury scores. c The lung W/D weight ratio. d MPO activity in lung tissues. e TNF-α, IL-1β, and IL-6 level in BALF. f Fe²⁺ level. g MDA level. h GSH level. i GPX4 activity. j GPX4 mRNA expression. k GPX4 protein expression. ^#P < 0.05 vs. LPS group, and ^&P < 0.05 vs. Fe+LPS group