Fig. 7.

PX inhibits ferroptosis via Keap1-Nrf2/HO-1 pathway. BEAS-2B cells are co-treated with 40 μg/ml of PX and the HO-1 inhibitor SnPP or Nrf2 inhibitor ML385 in the presence of LPS for 24 h. Then cell ferroptosis and inflammation are detected. a Cell viability determined by MTT assay. b Cell death detected by FDA staining kit. c Fe²⁺ level determined using Iron Assay Kit. d MDA level detected via the MDA Assay Kit. e GSH level detected by GSH assay kit. f GPX4 activity measured by GPX4 ELISA kit. g GPX4 mRNA expression determined by RT-PCR. h GPX4 protein expression detected by western blot assay. i TNF-α, IL-1β, and IL-6 level in cell culture supernatants determined via ELISA Kits. ^*P < 0.05 vs. ctrl group, ^#P < 0.05 vs. LPS group, and ^&P < 0.05 vs. PX(40) + LPS group