TABLE 2.
Strains and plasmids used in this study.
| Strains or plasmids | Description | Sources or references |
| Strains | ||
| Lysobacter antibioticus | ||
| OH13 | Wild-type strain | Zhao et al., 2016 |
| OH13(Plex-GFP) | OH13 with lex promoter driving gfp expression | This study |
| △LaPhzB | OH13 with deletion in LaPhzB | Zhao et al., 2016 |
| △LaPhzB(Plex-GFP) | △LaPhzB with lex promoter driving gfp expression | This study |
| △lexA | OH13 with deletion in lexA | This study |
| △lexB | OH13 with deletion in lexB | This study |
| △lexC | OH13 with deletion in lexC | This study |
| △lexABC | OH13 with deletion in lexABC | This study |
| △lexR | OH13 with deletion in lexR | This study |
| △lexA(pBBR) | △lexA carrying pBBR1-MCS5 vector, Gmr | This study |
| △lexA(pBBR-lexA) | △lexA carrying pBBR-lexA vector, Gmr | This study |
| △lexB(pBBR) | △lexB carrying pBBR1-MCS5 vector, Gmr | This study |
| △lexB(pBBR-lexB) | △lexB carrying pBBR-lexB vector, Gmr | This study |
| △lexABC(pBBR) | △lexABC carrying pBBR1-MCS5 vector, Gmr | This study |
| △lexABC(pBBR-lexABC) | △lexABC carrying pBBR-lexABC vector, Gmr | This study |
| Escherichia coli | ||
| DH5α | F- φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk-,mk +) phoA supE44 λ-thi-1 gyrA96 | TransGen, China |
| Plasmids | ||
| pJQ200SK | Suicide cloning vector, Gmr | Quandt and Hynes, 1993 |
| pJQ200SK-lexA | pJQ200SK with two flanking fragments of lexA | This study |
| pJQ200SK-lexB | pJQ200SK with two flanking fragments of lexB | This study |
| pJQ200SK-lexC | pJQ200SK with two flanking fragments of lexC | This study |
| pJQ200SK-lexABC | pJQ200SK with two flanking fragments of lexABC | This study |
| pJQ200SK-lexR | pJQ200SK with two flanking fragments of lexR | This study |
| pJQ200SK-Plex-gfp | pJQ200SK with two flanking fragments of lexA start site and gfp | This study |
| pBBR1-MCS5 | Broad host range cloning vector, lacZ, Gmr | Kovach et al., 1995 |
| pBBR-lexA | pBBR1-MCS5 carrying ORF of lexA and its promoter, Gmr | This study |
| pBBR-lexB | pBBR1-MCS5 carrying ORF of lexB and its promoter, Gmr | This study |
| pBBR-lexABC | pBBR1-MCS5 carrying ORF of lexABC and its promoter, Gmr | This study |