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. 2021 Mar;23(3):358–371. doi: 10.1016/j.jmoldx.2020.12.003

Table 2.

Characteristics of the Participants and Their Tumors That Were Included in This Study

SLS ID Sex Age at diagnosis, years Tumor ID Tumor type MMR IHC loss MSI status Family cancer history (MMR IHC result) Clinical criteria Final classification Evidence supporting classification
C1 F 19 CRC MSH2/MSH6 MSI-H Lynch MSH2: c.212-478T>G (germline)
C2 M 37 CRC MSH2/MSH6 MSI-H Lynch MSH2 deletion of exon 6 (germline)
SLS1 F 52 T1 CRC: adenocarcinoma of transverse colon MLH1/PMS2 NT Sister CRC (normal) at 42 years, breast at 53 years None Double MLH1 somatic MLH1: c.879C>G p.Tyr293Ter (somatic)
MLH1: c.1975C>T p.Arg659Ter (somatic)
SLS2 M 51 T2A Kidney MLH1/PMS2 NT None None NC
51 T2B Colonic tubular adenoma Normal NT
SLS3 M 45 T3 CRC: adenocarcinoma of transverse colon MLH1/PMS2 MSI-H Sister CRC at 40 years; brother CRC (normal) at 45 years, pancreas at 60 years; brother kidney (normal) at 57 years, prostate at 63 years; brother brain at 59 years Revised Bethesda Single MLH1 somatic MLH1: c.332C>T p.Ala111Val (somatic)
SLS4 F 68 T4A Breast Normal MSS Brother CRC at unknown age; sister CRC at 72 and 77 years; sister CRC (normal) at 67 and CRC (MLH1/PMS2 loss) at 78 years None Double MLH1 somatic Multiple somatic MLH1 variants including truncating mutation MLH1: c.1036C>T p.Gln346Ter, MMRd not present across all tumors
78 T4B Colonic tubulovillous adenoma MLH1/PMS2 NT
SLS5 F 42 T5 CRC: adenocarcinoma of transverse colon MLH1/PMS2 MSI-H Mother CRC at 45 years; father CRC at 54 years Amsterdam II Double MLH1 somatic MLH1: c.1989+1G>A (somatic)
MLH1: LOH (somatic)
SLS6 F 41 T6A CRC NT NT None Revised Bethesda Lynch MLH1: c.1958T>G p.Leu653Arg (germline)
53 T6B CRC: adenocarcinoma of transverse colon MLH1/PMS2 NT
53 T6C CRC MLH1/PMS2 NT
76 T6D Sebaceous carcinoma MLH1/PMS2 NT
SLS7 M 66 T7A CRC: adenocarcinoma of rectum MSH2/MSH6 MSI-H Daughter CRC at 45 years, mesothelioma (normal) at 48 years; sister endometrial at 37 years; brother bladder at 81 years; sister lung at 58 years Amsterdam II Double MSH2 somatic MSH2: c.1351C>T p.Gln451Ter (somatic)
79 T7B Colonic tubular adenoma Normal NT MSH2: c.1204C>T p.Gln402Ter (somatic)
SLS8 M 62 T8A Tubulovillous adenoma of rectum MSH2/MSH6 MSI-H Mother CRC at 93 years None Double MSH2 somatic MSH2: c.859G>T p.Gly287Ter (somatic)
62 T8B CRC Normal NT MSH2: c.2465_2466delinsAG p.Cys822Ter (somatic)
75 T8C Prostate NT NT
SLS9 F 54 T9A Duodenum MSH6 NT Daughter ovarian at 40 years and endometrial at 40 years None Double MSH2 somatic Multiple MSH2 somatic mutations, not shared with first-degree relative, MMRd not present across all tumors
58 T9B CRC: mucinous adenocarcinoma of ascending colon MSH2/MSH6 NT
67 T9C Brain Normal NT
SLS10 F 40 T10A Ovary MSH2/MSH6 MSI-H Double MSH2 somatic Multiple MSH2 somatic mutations, not shared with first-degree relative, mutational signatures 14 and 20 and POLD1 somatic mutation supporting somatic etiology
40 T10B Endometrium MSH2/MSH6 NT
SLS11§ F 40 T11A CRC: mucinous adenocarcinoma of caecum MSH2/MSH6 NT Daughter CRC at 35 years; brother CRC at 29 years and 46 years; brother CRC at 59 years Amsterdam I Lynch MSH2: 9.5 Mb Inversion ex1-7 (germline)
51 T11B Breast NT NT
SLS12§ F 35 T12 CRC: adenocarcinoma of sigmoid colon MSH2/MSH6 MSI-H Lynch MSH2: 9.5 Mb Inversion ex1-7 (germline)
SLS13 F 41 T13 CRC: adenocarcinoma of transverse colon MSH2/MSH6 NT None Revised Bethesda Single MSH2 somatic MSH2: c.2006-3T>G (somatic)
SLS14 F 66 T14 Endometrium MSH2/MSH6 NT Brother CRC at 63 years none Double MSH2 somatic MSH2: c.1276+2delT (somatic)
MSH2: c.2131C>T p.Arg711Ter (somatic)

Family Cancer History, cancer in first degree relatives only shown; MMR IHC results shown in parentheses.

F, female; M, male; CRC, colorectal cancer; MMR, mismatch repair; MSI, microsatellite instability; MSI-H, microsatellite instability-high; MSS, microsatellite stable; NT, not able to be tested; SLS, suspected Lynch syndrome.

Positive controls. Control 1: intronic mutation disrupting splicing. Control 2: deletion of exon 6 in MSH2.

Final Classification—classification for each SLS case after germline whole-genome sequencing and tumor sequencing analysis detailed in this study. Double and single somatic mutation classification considered to be non-Lynch syndrome and of sporadic etiology. NC, tumor failed testing and therefore no classification could be achieved.

Sister developed two CRCs: one at age 67 years with normal MMR protein expression and one at age 78 years with loss of MLH1/PMS2 protein expression resulting from MLH1 promoter hypermethylation in the tumor.

Relative pair: SLS10 is the daughter of SLS9.

§

Relative pair: SLS12 is the daughter of SLS11.