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To quantify the survival potential of CNTF, retinal cells grown in defined medium +CNTF for 72 h were double labeled with annexin V–FITC and propidium iodide, before subjecting to FACS analysis on day 6. Note the greater area of overlap (blue annexin V over red propidium iodide) in the control vs. cells treated with CNTF; in the lower panel, note that the blue line has moved to the right, reflecting an increase in the number of viable cells.
