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. Author manuscript; available in PMC: 2021 Mar 3.
Published in final edited form as: In Vitro Cell Dev Biol Anim. 2010 Apr 29;46(7):635–646. doi: 10.1007/s11626-010-9319-x

Figure 5.

Figure 5.

Ciliary neurotrophic-mediated differentiation phase contrast microscopy. Retinal cells exposed to CNTF (20 ng/ml) in defined medium were photographed at various times. Untreated cells (A, E, I). Panel 1, cells treated with bFGF 3 h+CNTF 24 h (b). Note the photoreceptor-like structures generated in response to bFGF (arrows). Cells treated with bFGF followed by CNTF (C, D). Neurons are mostly rods and cones at 48 and 72 h (arrows). Panel 2, cells treated with CNTF 3 h+bFGF 24 h (F). Note the multipolar neurons generated in response to CNTF. Cells treated with CNTF followed by bFGF (G, H)—mostly multipolar neurons are seen at 48 and 72 h. Panel 3, cells treated simultaneously with bFGF and CNTF (J, K, L). Cells seem to undergo multiple rounds of division before differentiating into multipolar neurons (arrows). Control cells labeled with BrdU for 3 h (M). Cells treated with CNTF (N). Note the cluster of cells undergoing multiple rounds of division. Cluster of dividing cells in (N) differentiating into multipolar neurons (O) arrows.