Table 2.
Advantages and disadvantages of current techniques in detection of SARS-CoV-2.
Tests | Analysis of Time | Advantages | Disadvantages | Ref |
---|---|---|---|---|
High-throughput genome sequencing | Around 1–2 weeks | Sequencing of fully genome | Need an expensive equipment | Soon et al. (2013) |
RT-PCR | About of 1–1.5 h | Good specificity | False-negative test results, lower sensitivity than CT scan in some patients, need a complex equipment | (Corman et al., 2020; Wikramaratna et al., 2020) |
RT-qPCR | About of 1–1.5 h | Highly sensitive technique for the detection and quantification of specific nucleic acids | Time-consuming, False-negative test results | Li et al. (2020a) |
LAMP | About 30min | LAMP is quick and does not need costly reagents or appliance, cost-effective and high specificity. high specificity, efficiency, and rapidity, diagnostic sensitivity >95%. | Primer design is complex, unable to perform multiplex amplification, need a non-specific dyes and false positive results | (Khan et al., 2018; Vijay J. Gadkar et al., 2018) |
ELISA | About 2 h with kit | High sensitivity (20–80%) | Antibody instability, high cost to synthesis antibody, IgG and IgM production takes place two weeks after the onset of infection | (Zhao et al., 2020a; Sakamoto et al., 2018) |
Computed tomography (CT) | Rapid analysis | Rapid detection assay with 97% sensitivity | Low specific | Ai et al. (2020) |