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. 2021 Mar 3;21:100415. doi: 10.1016/j.scp.2021.100415

Table 2.

Advantages and disadvantages of current techniques in detection of SARS-CoV-2.

Tests Analysis of Time Advantages Disadvantages Ref
High-throughput genome sequencing Around 1–2 weeks Sequencing of fully genome Need an expensive equipment Soon et al. (2013)
RT-PCR About of 1–1.5 h Good specificity False-negative test results, lower sensitivity than CT scan in some patients, need a complex equipment (Corman et al., 2020; Wikramaratna et al., 2020)
RT-qPCR About of 1–1.5 h Highly sensitive technique for the detection and quantification of specific nucleic acids Time-consuming, False-negative test results Li et al. (2020a)
LAMP About 30min LAMP is quick and does not need costly reagents or appliance, cost-effective and high specificity. high specificity, efficiency, and rapidity, diagnostic sensitivity >95%. Primer design is complex, unable to perform multiplex amplification, need a non-specific dyes and false positive results (Khan et al., 2018; Vijay J. Gadkar et al., 2018)
ELISA About 2 h with kit High sensitivity (20–80%) Antibody instability, high cost to synthesis antibody, IgG and IgM production takes place two weeks after the onset of infection (Zhao et al., 2020a; Sakamoto et al., 2018)
Computed tomography (CT) Rapid analysis Rapid detection assay with 97% sensitivity Low specific Ai et al. (2020)