Figure 1.

Overview of digitization‐enhanced CRISPR/Cas‐assisted one‐pot virus detection (deCOViD). SARS‐CoV‐2 RNA and inactivated SARS‐CoV‐2 virus can be detected by a single‐step CRISPR/Cas12a‐assisted reverse transcription recombinase polymerase amplification (RT‐RPA) assay, which produces DNA amplicons from RNA targets to activate Cas12a‐based cleavage of fluorogenic reporters in a single step. Importantly, compared to microliter‐scale bulk assay, deCOViD takes advantage of assay digitization to elevate the RNA concentration in sub‐nanoliter digital reaction wells and facilitate rapid amplification, leading to faster detection time for low target concentrations, higher signal‐to‐background (S/B) ratio, wider dynamic range, and better sensitivity.