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. 2021 Feb 24;14(2):dmm046300. doi: 10.1242/dmm.046300

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Aberrant processing of NOTCH3^L1519P. (A) Schematic overview of Notch proteolytic processing. (B) Expression of the full-length (FL), transmembrane intracellular domain (TMIC) and Notch extracellular truncated (NEXT)/Notch intracellular domain (NICD) forms was analyzed by western blotting of cell extracts from HEK 293T ΔN1-3 cells (Control) or HEK 293T ΔN1-3 cells expressing wild-type (WT) and NOTCH3^L1519P (L1519). β-actin levels were used as a loading control (n=3). Relative levels of TMIC/FL were quantified by ImageJ from three experiments and analyzed by paired Student's t-test, **P<0.01. ECD, extracellular domain; EGF, epidermal growth factor; NRR, negative regulatory region.