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. 2021 Feb 24;14(2):dmm046300. doi: 10.1242/dmm.046300

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Lack of cell surface expression and increased retention of NOTCH3^L1519P in the endoplasmic reticulum (ER). (A,B) Confocal images of wild-type and NOTCH ^L1519P-expressing cells without (A) and with (B) permeabilization. Na,K-ATPase was used as a membrane marker. (C) Immunocytochemistry for NOTCH3 ECD (green) and the ER marker calnexin (red) from permeabilized cells; the plot shows the Pearson's correlation coefficients for the colocalization analysis. (D) Immunocytochemistry for NOTCH3 ECD (red) and the Golgi marker giantin (green); the graph shows the Pearson's correlation coefficient analysis (n=3). Significance was calculated using unpaired Student's t-test, ***P<0.001; ns, not significant. Scale bars: 20 µm.