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. 2021 Feb 24;14(2):dmm046300. doi: 10.1242/dmm.046300

Fig. 7.

Fig. 7.

PDGFRB IMF mutations are gain-of-function mutations. (A) Analysis of phosphorylation levels from different PDGRFB mutants after stimulation with PDGF-BB, as indicated. (B) Quantification of phosphorylation of specific tyrosine residues (Y751, Y771, Y1009, Y1021) in the various PDGFRB mutants, as indicated (n=3). (C) Analysis of phosphorylation levels of PDGFRB, SHP2, Akt, p42/44 MAPK and eIF4E (as control) at different time points after PDGF-BB stimulation (0, 5, 15 and 60 min), as indicated. ***P<0.001 compared to the stimulated WT control (black bars); #P<0.05 and ###P<0.001 compared to unstimulated WT control (unpaired two-tailed Student's t-test). KD, knockdown.