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. 2021 Feb 17;12:628621. doi: 10.3389/fphar.2021.628621

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Copyright © 2021 Zhai, Zhang, Hei, Jin, Han, Ko, Yu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Isorhamnetin suppresses GBC cell proliferation in vitro. (A) Chemical structure of isorhamnetin. (B) The cell viability of GBC cells assessed using CCK-8 assay after isorhamnetin treatment (0, 40, 60, 80, and 100 μM) for 24, 48, and 72 h. (C) Colony formation assay of NOZ and GBC-SD cells exposed to isorhamnetin (0, 40, 60, , and 80 μM) for 10 days. (D) The colony numbers (>100 cells/colony) were calculated manually. *p < 0.05, ***p < 0.001, ****p < 0.0001 relative to the control group.