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. Author manuscript; available in PMC: 2021 Mar 3.
Published in final edited form as: Cell Rep. 2020 Nov 3;33(5):108330. doi: 10.1016/j.celrep.2020.108330

Figure 2. Absence of C4A Leads to Impaired Regulation of Autoreactive B Cells in Periphery.

Figure 2.

(A and B) Analysis of autoreactive B cell population (B220+ Id+) in peripheral blood of C4A 564Igi (n = 5) and C4B 564Igi (n = 8) by flow cytometry. (A) Representative dot plots. (B) Percentage of Id+ B cells within the total B220+ B cell population. Means ± SEMs; Mann-Whitney test; 1 dot represents the average percentage for 1 strain.

(C–E) Analysis of autoreactive Id+ B cells within the total B220+ B cell population in secondary lymphoid organs by flow cytometry.

(C) Proportion of splenic Id+ B cells within the total B220+ B cell population from 8, 15, and 20 wo C4A and C4B 564Igi mice. Means ± SEMs; Mann-Whitney test; each dot represents the average of n > 8 mice.

(D) Proportion of Id+ B cells within the total B220+ B cell population in spleen (top panel) and skin-draining LNs (bottom panel) from 8 and 15 wo C4A, C4B, C4+/+, and C4−/− 564Igi. Means ± SEMs; 1-way ANOVA with Tukey’s test; each dot represents 1 mouse; n > 4 for each strain.

(E) Gating strategy to discern immature (i, B220+ AA4.1+), transitional (t, B220+ AA4.1int), and mature (m, B220+ AA4.1−) splenic B cell subpopulations.

(F) Proportions of Id+ B cells within immature, transitional, and mature splenic B cell subpopulations of 15 wo C4A, C4B, C4+/+, and C4−/− 564Igi. Means ± SEMs; 1-way ANOVA with Tukey’s test; each dot represents 1 mouse; n > 6.

*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. See also Figure S2.