KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Monoclonal Rat anti-Mouse C4 (clone 16D2) | (Gift of E. Kremmer, Munich, Germany) | N/A |
| Polyclonal Rabbit anti-Human C4c | Dako | F0169 |
| Goat IgG fraction to mouse complement C3 | MP Biomedicals | Cat# ICN55463, RRID:AB_2334481 |
| Polyclonal Donkey anti-Goat IgG Ax488 | Life technology | Cat# A-11055, RRID:AB_2534102 |
| Polyclonal Goat anti-Rat IgG Ax488 | Life technology | Cat# A-11006, RRID:AB_2534074 |
| Polyclonal Goat anti-Rabbit IgG (H+L), Human/Mouse absorbed-AP | Southern biotech | Cat# 4050-04, RRID:AB_2795954 |
| Polyclonal Goat anti-Mouse IgG (H+L), Human absorbed-HRP | Southern biotech | Cat# 1031-05, RRID:AB_2794307 |
| Polyclonal Goat anti-Mouse IgG, Human absorbed-AP | Southern biotech | Cat# 1030-04, RRID:AB_2794293 |
| Polyclonal Goat anti-Mouse IgG2a, Human-absorbed-AP | Southern Biotech | Cat# 1080-04, RRID:AB_2692322 |
| IgG from murine serum | Sigma | Cat# I5381, RRID:AB_1163670 |
| Purified Mouse Ig2a | Southern biotech | Cat# 0103-01, RRID:AB_2736850 |
| Monoclonal Rat anti-Mouse/Human B220 APC | Biolegend | Cat# 103212, RRID:AB_312997 |
| Monoclonal Rat anti-Mouse/Human B220 PerCP/Cy5.5 | Biolegend | Cat# 103236, RRID:AB_893354 |
| Monoclonal Rat anti-Mouse/Human B220 Pacblue | Biolegend | Cat# 103227, RRID:AB_492876 |
| Monoclonal Rat anti-Mouse CD3 FITC | Biolegend | Cat# 100203, RRID:AB_312660 |
| Monoclonal Rat anti-Mouse CD3 PE | Biolegend | Cat100308, RRID:AB_312673 |
| Monoclonal Rat anti-Mouse CD4 FITC | Biolegend | Cat# 100405, RRID:AB_312690 |
| Monoclonal Rat anti-Mouse CD4 PB | Biolegend | Cat# 100428, RRID:AB_493647 |
| Monoclonal Rat anti-Mouse CD11b APC | Biolegend | Cat# 101212, RRID:AB_312795 |
| Monoclonal Rat anti-Mouse CD21/35 (clone 7E9) PB | Biolegend | Cat# 123414, RRID:AB_2085158 |
| Monoclonal Rat anti-Mouse CD38 PeCy7 | Biolegend | Cat# 102718, RRID:AB_2275531 |
| Monoclonal Rat anti-Mouse CD93 (AA4.1) PerCP/Cy5.5 | Biolegend | Cat# 136511, RRID:AB_10645333 |
| Monoclonal Rat anti-Mouse CD138 PE | Biolegend | Cat# 142503, RRID:AB_10915989 |
| Monoclonal Rat anti-Mouse CD169 FITC | Biolegend | Cat# 142406, RRID:AB_2563107 |
| Monoclonal Rat anti-Mouse CD185 (CXCR5) Biotin | Biolegend | Cat# 145510, RRID:AB_2562126 |
| Monoclonal Rat anti-Mouse CD206 PeCy7 | Biolegend | Cat# 141719, RRID:AB_2562247 |
| Monoclonal Rat anti-Mouse CD279 (PD-1) APC | Biolegend | Cat# 135210, RRID:AB_2159183 |
| Monoclonal Rat anti-Mouse CD357 (GITR) PeCy7 | Biolegend | Cat# 126317, RRID:AB_2563385 |
| Monoclonal Rat anti-Mouse F4/80 FITC | Biolegend | Cat# 123108, RRID:AB_893502 |
| Monoclonal Rat anti-Mouse GL7 PB | Biolegend | Cat# 144614, RRID:AB_2563292 |
| Monoclonal Rat anti-Mouse IgD PB | Biolegend | Cat# 405712, RRID:AB_1937244 |
| Monoclonal Rat anti-Mouse IgM PE | Biolegend | Cat# 406507, RRID:AB_315057 |
| Monoclonal Mouse anti-Mouse IgMa PE | Biolegend | Cat# 408608, RRID:AB_940545 |
| Monoclonal Mouse anti-Mouse IgMb FITC | Biolegend | Cat# 406206, RRID:AB_315039 |
| Monoclonal Mouse anti-idiotype (clone 9D11) | Chatterjee et al., 2013 | N/A |
| Biological Samples | ||
| EA cells | Complement technology | B202 |
| C4−/− guinea pig serum | Complement technology | A305 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Ro60 Protein | Diarect | 17400 |
| BPI Protein | Diarect | 19200 |
| CTGF Protein | R&D system | 9190-CC |
| Smd2 protein | Diarect | 11900 |
| C1q protein | Meridian Life Science | A01670H |
| APRIL protein | PEPROTECH | 310-10C |
| Ebioscience™ Fixable viability Dye eFluor™ 780 | Invitrogen | 65-0865-14 |
| AnnexinV FITC | Biolegend | 640905 |
| Propidium Iodide | Sigma | P4170 |
| Streptavidin-APC | Biolegend | 405207 |
| Streptavidin-PE | Biolegend | 405204 |
| pH-Rhodo™ Red, SE | Life technology | P36600 |
| Thioglycollate | BD | 211716 |
| Tamoxifen | Sigma | T5648 |
| Sunflower seed oil | Spectrum chemical | 51929 |
| Streptavidin-coated microspheres | Bangs Laboratories | CP01N |
| Experimental Models: Organisms/Strains | ||
| Mouse: 564Igi: 564 HiKi | Berland et al., 2006 | N/A |
| Mouse: AIDCre.ERT2.eYFP: AicdaCreERT2 flox-STOP-flox-EYFP | Dogan et al., 2009 | N/A |
| Mouse: C4−/−: C57BL/6.C4−/− | Fischer et al., 1996 | N/A |
| Mouse: mC4A: C57BL/6.mC4A/A | This paper | N/A |
| Mouse: mC4B: C57BL/6.mC4B/B | This paper | N/A |
| Oligonucleotides | ||
| Primers for genotyping | This paper | Table S1 |
| Primers for ddPCR | This paper | Table S4 |
| Software and Algorithms | ||
| ImageJ | Rasband WS, ImageJ, NIH, Bethesda, Maryland, USA | https://imagej.nih.gov/ij/ |
| CellProfiler | Lamprecht et al., 2007 | https://cellprofiler.org/ |
| Other | ||
| Antigens used in autoantigen array | This paper | Table S6 |