FIG 6.

Effect of itraconazole, lopinavir (LPV), or a combination of the two drugs on the mRNA expression levels of five selected genes involved in glucose transport and efflux in C. auris. Exponentially grown C. auris AR0390 cells were treated with either LPV (10 μg/ml), ITC (1 μg/ml), or a combination of the two drugs for 3 h. Following treatment, cells were harvested and lysed, and RNA was extracted. The expression of genes encoding glucose transport (HGT6 and HGT8) and the azole resistance-related efflux genes (CDR1, CDR2, and MDR1) was internally normalized to ACT1 and compared to that of the untreated control. Asterisks indicate significant changes in expression of the examined genes (at least 2-fold differences, up or down) relative to that of the untreated control. The results are presented as means ± SD.