TABLE 1.
Sequences, strains, and plasmids examined in this study
| Strains and plasmids | Characteristicsa | Reference or source |
|---|---|---|
| Strains | ||
| E. coli EC623771 | Fosfomycin-resistant isolate | CANWARD |
| E. coli EC623772 | Fosfomycin-resistant isolate | CANWARD |
| E. coli EC623773 | Fosfomycin-resistant isolate | CANWARD |
| E. coli BW25113 | F–, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ–, rph-1, Δ(rhaD-rhaB)568, hsdR514 | NBRPb |
| E. coli pMS119EH | E. coli BW25113 transformed with pMS119EH | This study |
| E. coli FosA3 | E. coli BW25113 transformed with pMS-FosA3 | This study |
| E. coli FosA8 | E. coli BW25113 transformed with pMS-FosA8 | This study |
| E. coli FosA7.5WT | E. coli BW25113 transformed with pMS-FosA7.5WT | This study |
| E. coli FosA7.5Q86E | E. coli BW25113 transformed with pMS-FosA7.5Q86E | This study |
| E. coli FosA7.5W92G | E. coli BW25113 transformed with pMS-FosA7.5W92G | This study |
| Plasmids | ||
| pMS119EH | NruI-NdeI deletion of pJF119EH vector | 22 |
| pMS-FosA3 | C-terminus His6-tagged fosA3 EC623771 gene cloned in pMS119EH | This study |
| pMS-FosA8 | C-terminus His6-tagged fosA8 EC623773 gene cloned in pMS119EH | This study |
| pMS-FosA7.5WT | C-terminus His6-tagged fosA7.5 WP_000941933.1 gene cloned in pMS119EH | This study |
| pMS-FosA7.5Q86E | C-terminus His6-tagged fosA7.5 EC623772 gene cloned in pMS119EH | This study |
| pMS-FosA7.5W92G | C-terminus His6-tagged fosA7.5 WP_094163054.1 gene cloned in pMS119EH | This study |
a
His6, hexahistidine; C-terminus, carboxy-terminus.
b
National BioResource Project, Keio Collection (23).