FIG 1.

Diet-induced model of murine T2D, M. tuberculosis (Mtb) infection, and treatments. (a) Six- to 8-week-old C57BL/6 male mice were fed EDD and standard rodent diet (SD; control mice) for 30 weeks to induce murine T2D. Following dietary intervention, mice were assessed for body weight (b), fasting blood glucose levels (c), and glucose tolerance (d). (d) Glucose tolerance test was performed by measuring glucose concentrations at 15, 30, 60, and 120 min after intraperitoneal glucose administration (2 g/kg) and calculating area under the curve (AUC). (a) Diabetic and nondiabetic control mice were infected with a very low aerosol dose (10–20 CFU) of M. tuberculosis H37Rv. Seven days p.i., MET (500 mg/kg), INH (10 mg/kg), and MET+INH were administered in drinking water. Results are presented as individual data points (b–d) and pooled data means ± SEM (d) from 2 pooled independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, by Student's t test.