FIG 2.

Effect of shCD5 treatment on kidney cytokine and leukocyte infiltration levels of C. albicans-infected CD1 mice. (A) Kidney cytokine levels at 72 h postinfection of CD1 mice infected with C. albicans (3 × 10³ CFU/g, i.v.) and treated (i.v.) with a single 1.25-mg/kg dose of HSA (n = 14) or shCD5 (n = 14) 18 h later. Represented are fold inductions with regard to noninfected controls (basal, n = 4). (B) Kidney relative cytokine mRNA expression at 48 h postinfection of CD1 mice infected with C. albicans (3 × 10³ CFU/g, i.v.) and treated (i.v.) with a single 1.25-mg/kg dose of HSA (n = 9) or shCD5 (n = 7) 18 h later. Represented is the relative expression with regard to noninfected controls (basal, n = 4). (C) Total leukocyte (CD45⁺), B (B220⁺ CD3⁻), T (CD3⁺ B220⁻), natural killer (NK; NK1.1⁺ CD3⁻), granulocyte (Gr; Gr-1⁺ SSC^hi), conventional dendritic (cDC; CD11c⁺ B220⁻), and macrophage (Mϕ; CD11c⁻ Gr-1^low SSC^low) cell numbers assessed by flow cytometry from the same mice as those depicted in panel A. Statistical differences between groups were analyzed by Mann-Whitney test or Kruskal-Wallis and Dunn’s test. *, P ≤ 0.05; **, P ≤ 0.01.