FIG 6.

AME-induced gentamicin resistance is impacted by the presence of the MexXY-OprM efflux pump. Gentamicin resistance levels of P. aeruginosa PA14 and mutants thereof. All strains were transformed with the pSEVA621 vector, harboring a gentamicin acetyltransferase (AME⁺) (35). The ΔmexAB AME⁺ strain was complemented with pSEVA634::oprM (ΔmexAB AME⁺/+oprM). pSEVA634 harbors the same acetyltransferase as pSEVA621. Red line depicts the median resistance level of at least 4 biological replicates. A P value of < 0.05 represents statistical significance (Wilcoxon rank sum test).