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. 2020 Mar 26;106(3):692–700. doi: 10.3324/haematol.2019.236513

Figure 2.

Figure 2.

Microenvironmental triggering induces stem cell factor in chronic lymphocytic leukemia cells. (A) Comparison of stem cell factor (SCF)+ cells (flow cytometry for viable/SCF+ chronic lymphocytic leukemia [CLL] cells) from IG-unmutated CLL (U-CLL) and IG-mutated (M-CLL) cases, untreated (Control) cases and cases treated with IgM/IgG. (B) Comparison of SCF+ cells (flow cytometry for viable/SCF+ CLL cells) from U-CLL and M-CLL cases, untreated (Control) cases and cases treated with CpG. (C) Comparison of secreted SCF (pg/mL; determined by enzyme-linked immunosorbent assay in cellular supernatants) from U-CLL cells, untreated (Control) and CpG-treated cells. (D, E) Comparison of proliferating cells (Ki-67+) (D) and SCF+ cells (%) (E) in untreated (Control) and CpG/CD40L-treated CLL cases, as determined by flow cytometry. (F) The majority of proliferating (Ki-67+) CLL cells were also SCF+ as determined by flow cytometry. (G) Lymph node (left) and the corresponding bone marrow biopsy (right) from the same CLL patient showing CLL invading cells with aberrant SCF expression. (H) Reactive, non-CLL lymph node (left) exhibiting SCF positivity mostly in mantle zones but not in germinal centers and reactive, non-CLL bone marrow (right) exhibiting scant SCF positivity in scarce immune and endothelial cells. Interconnected dots represent one case in two different conditions while bars represent median values. The Wilcoxon P test was applied to evaluate statistical significance; *P<0.05, **P<0.01, ***P<0.001. FD: fold difference.