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. 2020 Apr 17;106(3):759–769. doi: 10.3324/haematol.2019.225987

Figure 1.

Figure 1.

Generation of mice with megakaryocyte- and platelet-specific deletion of Slc35a1. (A) The function of Slc35a1-encoded CMP-sialic acid transporter (CST). (B) Targeting strategy for conditional deletion of the Slc35a1 gene. Diagram of wild-type (WT) (Slc35a1), loxP site-flanked (Slc35a1f/f), and null (Slc35a1–/–) alleles of Slc35a1. Arrowheads indicate the position of loxP. (C) Genotyping polymerase chain reaction of tail genomic DNA from offspring was used to identify Plt Slc35a1–/– mice. Lane 3 represents the genotype of Plt Slc35a1–/– mice. (D) Slc35a1 gene expression in WT and Plt Slc35a1–/– mice was determined by quantitative reverse transcriptase polymerase chain reaction using RNA extracted from bone marrow megakaryocytes (n=3 for each group).