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. 2020 Feb 20;106(3):838–846. doi: 10.3324/haematol.2019.236836

Figure 2.

Figure 2.

Cross-presentation of Dickkopf-1 (DKK1)3-76-long peptide (LP) efficiently primes DKK1-specific CD8+ T cells in vivo. Shown are CD8+ T-cell responses induced in HLA-A*0201-transgenic mice after immunization with DKK13-76-LP or HLA-A*0201-restricted P20 short peptide. (A) DKK1-P20-specific tetramer staining showing the frequency of DKK1 P20-specific CD8+ T cells in the spleen of a HLA-A*0201-transgenic mouse. Representative results from one of three mice are shown. (B) DKK1-P66v-specific tetramer staining showing the frequency of DKK1 P66v-specific CD8+ T cells in the spleen of an HLA-A*0201-transgenic mouse. These results indicate that DKK13-76-LP was cross-presented and efficiently primed DKK1-P66v-specific CD8+ T cells in HLA-A*0201-transgenic mice. Representative results from one of three mice are shown. (C) Cytolytic activity of CD8+ T cells isolated from mice immunized with DKK13-76 LP against unpulsed dendritic cells (DC) or DC pulsed with DKK13-76 LP or P20 short peptide. Representative results of three experiments are shown. (D) Cytolytic activity of CD8+ T cells isolated from mice immunized with DKK13-76 LP against U266 and ARP-1 human myeloma cell lines, and K562. Representative results of three experiments are shown. *P<0.05; **P<0.01.