FIG 3.

A. muciniphila and Amuc_1100 increase the secretion of the anti-inflammatory mediator IL-10 by BMM infected with P. gingivalis. BMM were exposed to the control (Us), P. gingivalis alone (Pg) (MOI, 20:1), A. muciniphila alone (Akk) (MOI, 40:1), A. muciniphila and P. gingivalis (Akk/Pg), Amuc_1100 alone (1 μg/ml or 10 μg/ml), or Amuc_1100 and P. gingivalis (Amuc_1100/Pg) (1 μg/ml or 10 μg/ml). TNF-α (A) and IL-10 (B) concentrations were determined after 8 h of stimulation. All values are represented as means ± SEM (n, 5/group). Results were analyzed by one-way ANOVA, with differences considered significant at a P value of ≤0.05. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001. (Asterisks indicate comparisons with P. gingivalis.)