FIG 5.

Increased expression of IL-10 and CXCL10 in the gingival tissues of mice exposed to A. muciniphila or Amuc_1100 correlated with reduced alveolar bone loss. Gingival tissues isolated at the endpoint of the gavage period were analyzed for mRNA expression. Mice were divided into the following groups: PBS (control), P. gingivalis (Pg), A. muciniphila (Akk), P. gingivalis and A. muciniphila (Akk/Pg), Amuc_1100 (1100), and Amuc_1100 and P. gingivalis (1100/Pg). The relative expression of mRNA was determined by quantitative real-time PCR using TaqMan assays. The fold change was calculated using control tissue as a baseline and β-actin as a normalizing control. All values are represented as means ± SEM (n, 6/group). Results were analyzed by Pearson’s correlation analysis, with differences considered significant at a P value of ≤0.05.