Figure 5.
Effects of PARP-1 inhibition on S100B-induced nitric oxide (NO) and matrix metalloproteinase-9 (MMP9) release. A. Representative MMP9 immunoblots prepared from the medium of microglial cultures treated for 24 hours with 200 ng/ml S100B, ± 5 μM veliparib. B. MMP9 immunoblots prepared from the medium of PARP-1−/− microglial cultures treated for 24 hours with 200 ng/ml S100B. C. Nitrite concentrations in medium of microglial cultures treated for 24 hours with 200 ng/ml S100B or 1 ng / ml LPS ± 5 μM veliparib. D. Nitrite concentrations in medium of PARP-1−/− microglial cultures treated for 24 hours with 200 ng/ml S100B. Cultures in (C) and (D) were also treated with 20 ng/ml IFN-γ. For all panels n = 3, ** P < 0.01.