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. 2020 Apr 30;106(3):903–907. doi: 10.3324/haematol.2020.251462

Figure 3.

Figure 3.

The effect of FAM122A silencing on phosphorylatios of PP2A substrates and MYC expression. (A) Acute myeloid leukemia (AML) cells with FAM122A silencing and reexpression (shFAM122A#1-FAM122A-6m) were subjected to western blot for detection of the indicated proteins. (B) The indicated cells were treated with or without 50 nM okadaic acid (OA) for 6 h (NB4 cells), or 20 nM OA for 12 h (U937 cells), and then western blotting was performed to examine protein levels. (C) The MYC mRNA expression level was examined in FAM122A knockdown AML cells. Data are means with the bar indicating the standard deviation in an independent experiment. ***P<0.001 compared with shScramble cells. (D, E) FAM122A knockdown NB4 cells, together with shScramble cells, were treated with or without 20 mΜ MG132 for 4 h (D), or cyclohexamide at a dose of 100 mg/mL at different time points (E), followed by western blot to examine the indicated proteins. CHX: cyclohexamide.