Figure 1.

Analysis of hematologic parameters and erythropoiesis of wild-type and Ncoa4-ko mice fed a standard diet. Ncoa4-ko and wild-type (wt) mice (of both genders) on a Sv129/J background were fed a standard diet until sacrifice at the age of 9 months. Complete blood counts were periodically determined. (A) Values are shown for red blood cell count, hemoglobin levels, mean corpuscular volume and mean corpuscular hemoglobin. (B) Gating strategy for the analysis of erythropoiesis. Viable cells (impermeable to propidium iodide) from bone marrow and spleen were analyzed for Ter119/CD44 expression. Ter119⁺ cells were gated and further analyzed with respect to forward scatter and CD44 surface expression for subpopulation composition. Five clusters were identified: proerythroblasts (I), basophilic erythroblasts (II), polychromatic erythroblasts (III), orthochromatic erythroblasts and immature reticulocytes (IV), and mature red cells (V).¹³ The complete statistical analysis is reported in Online Supplementary Table S3. (C) Percentage of Ter119⁺ cells on alive cells and subpopulation composition in the bone marrow and in the spleen of 3-month old wt and Ncoa4-ko mice. The complete statistical analysis is reported in Online Supplementary Table S4. (D) Colony numbers generated from 10⁴ bone marrow cells of 3- and 9-month old wt and Ncoa4-ko mice. Mean values of six animals per genotype are shown. Error bars indicate the standard error. Asterisks refer to statistically significant differences between age-matched wt and Ncoa4-ko mice. *P<0.05; ***P<0.005. RBC: red blood cells; Hb: hemoglobin; MCV: mean corpuscular volume; MCH: mean corpuscular hemoglobin; BM: bone marrow, SP: spleen; CFU-GM: colony-forming unit-granulocyte/monocyte; BFU-E: burst-forming unit-erythroid.