Table 1.

SAXS-derived parameters and experimental details

Instrument		SEC-SAXS at SWING beamline SOLEIL
q range (Å −1)		0.0022–0.62
Temperature (°C)		25°C
Sample	Rg(Å) Reciprocal Space	I(0) (cm−1/absorbance) Reciprocal Space	Rg(Å) Real Space	I(0) (cm−1/absorbance) Real Space	Dmax (Å)	Porod Volume estimate (Å3)	MW from Porod Volume/ 1.6 (kDa)	MW from sequence (kDa)
HsLa La-module	26.90 ± 0.40	00029 ± 1.1 10−5	26.97 ± 0.09	0.002876 ± 8.285 10−6	84	46400	29.0	22.3
HsLa La-module-U4 complex	23.82 ± 0.25	0.0022 ± 1.5 10−5	22.76 ± 0.08	0.002013 ± 6.678 ± 10 −6	69	40300	25.0	22.3
HsLaRP7 La-module	32.08 ± 1.02	0.013 ± 2.6 10−5	32.20 ± 0.10	0.01289 ± 2.965 10−5	101	58200	36.4	24.0
HsLaRP7 La-module-U4 complex	26.79 ± 0.30	0.024 ±3.5 10−5	27.40 ± 0.05	0.02369 ± 3.196 10−5	92	48000	30.0	24.0
HsLaRP6 85–300 La-module	27.18 ± 0.18	0.0045 ± 3 10−5	27.20 ± 0.07	0.004412 ± 1.539 10−5	81	51900	32.4	23.9
HsLaRP6 85–300 La-module-48ntSL complex	31.48 ± 0.13	0.0032 ± 1.8 10−6	31.60 ± 0.07	0.003138 ± 1.220 10−5	90	94000	58.8	39.8
48ntSL RNA	22.29 ± 1.77	0.0091 ± 9.9 10−6	22.47 ± 0.03	0.009076 ± 8.442 10−6	75	20400	12.8	14.4
HsLaRP4A La-module	22.52 ± 0.60	0.0012 ± 2.5 10−5	21.66 ± 0.16	0.001151 ± 8.423 10−6	68	27800	17.4	20.5
HsLaRP4B La-module	21.33 ± 0.29	0.0018 ± 2.8 10−5	21.37 ± 0.03	0.01821 ± 1.937 10−5	67	28900	18.1	21.6
Software employed
Primary reduction		Foxtrot
Data processing		ATSAS 2.8 and Scatter
Ab initio analysis		DAMMIF/DAMMIN
Validation and averaging		DAMAVER
Computation of model intensities		CRYSOL
3D graphic representation		PyMOL