Table 1.
Experimental parameters used to collect the NMR experiments
| Experiments | Dimension of acquired data | Spectral width (ppm) | NSa | d1 + aq (s)b | Spectrometer frequency (1H) (MHz) | ||||
|---|---|---|---|---|---|---|---|---|---|
| t1 | t2 | t3 | F1 | F2 | F3 | ||||
| 1H detected | |||||||||
| 1H-15N BEST-TROSY | 512 (15N) | 9676 (1H) | 41 | 15 | 16 | 0.5 | 1200 | ||
| BT-HNCACB | 96 (13C) | 90 (15N) | 6144 (1H) | 75 | 41 | 14 | 96 | 0.2 | 1200 |
| BT-HN(CO)CACB | 96 (13C) | 80 (15N) | 6144 (1H) | 75 | 41 | 14 | 96 | 0.2 | 1200 |
| HN(CA)CO | 128 (13C) | 128 (15N) | 4096 (1H) | 7 | 28 | 18 | 8 | 1.0 | 1200 |
| HNCO | 128 (13C) | 220 (15N) | 4096 (1H) | 7 | 28 | 18 | 4 | 1.0 | 1200 |
| 13C detected | |||||||||
| CON | 512 (15N) | 1024 (13C) | 34 | 31 | 32 | 1.6 | 700 | ||
| (HCA)CON | 220 (15N) | 1024 (13C) | 40 | 31 | 16 | 0.9 | 700 | ||
| (H)CACO | 330 (13C) | 1024 (13C) | 34 | 30 | 32 | 1.0 | 700 | ||
| (H)CBCACO | 476 (13C) | 1024 (13C) | 59 | 30 | 32 | 1.0 | 700 | ||
| (H)CBCACON | 128 (13C) | 96 (15N) | 1024 (13C) | 58 | 34 | 30 | 4 | 1.0 | 700 |
| (H)CBCANCO | 96 (13C) | 96 (15N) | 1024 (13C) | 58 | 34 | 30 | 16 | 1.0 | 700 |
aNumber of acquired scans
bRelaxation delay (acquisition time plus recovery delay d1)