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. 2021 Feb 25;10(2):bio050096. doi: 10.1242/bio.050096

Fig. 1.

Fig. 1.

Tspan18 expression in the cardiovascular system. The expression of Tspan18 and its paralogue Tspan12 was analyzed by RT-PCR in (A) human fetal tissues and (B) primary cultured endothelial cells and tumor cell lines. UASMC, umbilical artery smooth muscle cell; HUVEC, human umbilical vein endothelial cell; HUAEC, human umbilical artery endothelial cell; NC, negative control with no cDNA. (C) Quantitative RT-PCR analysis of Tspan18 expression at different times in zebrafish development (whole embryo). Expression was normalized to β–actin. (D) Whole-mount ISH of Tspan18 expression in the developing embryos. D is the cross section of C. DA, dorsal aorta; PCV, posterior cardinal vein; DLAV, dorsal longitudinal anastomotic vessels; ISV, intersegmental vessel; S, somites; NC, Notochord. All larvae lateral view unless indicated. Anterior is to the left. Scale bars:100 μm. All experiments (A–D) were repeated at least three times and similar results were obtained.