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. 2021 Feb 17;8:624166. doi: 10.3389/fmed.2021.624166

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Copyright © 2021 JAK, Zanirati, Rodrigues, Grahl, Krimberg, Pinzetta, Borém, Savi, Machado, Da Costa and Marinowic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Real-time PCR amplification, melt curves, and ssDNA sequencing of SARS-CoV-2 from different tissues of the newborn. (A) Amplification curve of SARS-CoV-2 in samples from the placenta, lung, heart, liver, and brain. (B) The melt curves showed a similar pattern for all SARS-CoV-2-positive amplifications detected in all tissues analyzed. (C) ssDNA sequencing of generated amplicons. The results obtained by sequencing the amplicons showed high homology with SARS-CoV-2 sequences. The homology analysis was performed using the entire genome sequences of copies of the SARS-CoV-2 virus from Russia (MT890462.1 RUS), USA (MT642254.1 USA), Italy (MT890669.1 ITA), China (MT079844.1 CHN), and Brazil (MT827074.1 BRA).