TABLE 1.

Summary of germline transmission of knocked-in sox3 alleles.

Tag-3′ homology arm length	Number of fish screened	Number of germline transmitted fish (F0 founders)	Rate of germline transmission	F0 founders with correct integration		Rate of F1 embryos with correct integration
				Rate	ID #
FLAGx3-300	37	7	19% (7/37)	3% (1/37)	#9	16% (8/50)
FLAGx3-50	28	8	29% (8/28)	21% (6/28)	#9	ND
					#16	85% (17/20)
					#19	5% (1/20)
					#20	ND
					#21	50% (12/24)
					#22	10% (2/20)
PAx3-300	30	5	17% (5/30)	3% (1/30)	#7	ND
PAx3-50	47	10	21% (10/47)	6% (3/47)	#21	55% (11/20)
					#25	ND
					#34	ND

ND, not determined.