Figure 2.

Asymptomatic HSV-1 infection accelerates the onset and increases the severity of EAE. (A) Schematic representation of the experimental design carried out in this study. (B) EAE was scored for each mouse after EAE induction, which was carried out 30–35 days post-HSV-1 infection. Mice were followed until day 21 post-EAE induction. The graph shows the means of disease scores ± SEM for mice mock-treated (blue circles), infected with WT HSV-1 (17syn+ strain, green squares) or inoculated with HSV-1 Δ34.5 (F strain, red triangles) in three independent experiments (n = 12/group). Data were analyzed using two-way ANOVA followed by Tukey's post-test; ****p < 0.0001, ***p < 0.001, and *p < 0.05.