Figure 6.

Animals inoculated with HSV-1 and treated to undergo EAE display increased numbers of neutrophils infiltrating the spinal cord. Mice were mock-treated, infected with WT HSV-1 (17syn+ strain) or inoculated with HSV-1 Δ34.5 (F strain). EAE was induced 4 weeks post-infection. At day 14 post-EAE induction, mice were perfused, and the spinal cords were harvested and processed to isolate immune cells infiltrating this tissue. Virus-inoculated and uninfected mice without EAE were included as controls. (A) Total lymphoid cells (left) and myeloid cells (right) infiltrating the spinal cords of mice induced to develop EAE. Values represent the means ± SEM of two independent experiments. Data were analyzed using Kruskal–Wallis and Dunn's multiple comparisons post-test; *p < 0.05 (n = 8/group). (B) Infiltrating CD4⁺ (left) and CD8⁺ (middle) T cells, or CD19⁺ B cells (right) from the spinal cord of mice induced to develop EAE and uninfected or inoculated with HSV-1, yet without EAE are plotted individually. (C) Infiltrating Ly6C⁺ (left) and Ly6G⁺ (middle) myeloid cells from the spinal cords of mice induced to develop EAE and uninfected or mice inoculated with HSV-1, yet without EAE are plotted individually. Data are means ± SEM of two independent experiments for the EAE group (n = 8/group) and without EAE group (n = 2–5/group). For the percentage of CD45^lo+/CD11b⁺/MHC-II⁺ activated microglia (right), the data are means ± SEM of n = 4/group. Data were analyzed using Kruskal–Wallis and Dunn's multiple comparisons post-test **p < 0.01, *p < 0.05, and n.s. non-significant. T-tests were used to compare animals inoculated with either, WT or Δ34.5 HSV-1, induced to develop EAE and their counterparts without EAE. (D) Representative FACS plots showing the distribution of infiltrating myeloid cells in the spinal cords. Live single cells were pre-gated on CD45⁺ and CD11b⁺ cells. Infiltrating CD45^hi+/CD11b⁺ myeloid cells were subdivided into neutrophils (Ly6G⁺) and monocytes (Ly6C⁺).