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. 2021 Feb 12;15:602888. doi: 10.3389/fncel.2021.602888

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Copyright © 2021 Tolomeo, Laterza, Grespan, Michielin, Canals, Kokaia, Muraca, Gagliano and Elvassore.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transient activation of lentiviral mediated Ngn2 expression does not allow PAX6 positive cell appearance in culture. (A) Experimental design. Note that dox was provided only for 6 h in culture (B) Representative images of hiPSCs with a transient activation of Ngn2 stained for pluripotency (OCT4, green) neuronal (βIIITUBULIN, red) and NSC (PAX6, red) markers (scale bar = 100 μm). (C) Percentage of OCT4, PAX6, and βIIITUBULIN positive cells over the total number of cells at Day 10. n = 3 independent replicates (mean ± SEM); ns, not significant.

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