FIGURE 4.

Liraglutide reduced high glucose-induced RGCs damages through mitophagy in vitro. As shown in the figure, RGCs were respectively treated with normal glucose (25 mmol/L), high glucose (65 mmol/L), high glucose (65 mmol/L), and liraglutide (10 nmol/L) for 24 h. Additionally, RGCs were co-treated with high glucose (65 mmol/L), liraglutide (10 nmol/L), and rapamycin for 24 h. (A) Relative LC3A/B and p62 expression was determined by Western blot. (B,C) Quantification of LC3A/B and p62. (D) Cell viability was detected with CCK-8 assay kits. Values are presented as mean ± SEM. n = 3 independent experiments. *p < 0.05, **p＜0.01, ***p < 0.001.