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. 2021 Feb 12;10:607231. doi: 10.3389/fonc.2020.607231

Figure 5.

Figure 5

MiR-140-3p directly targets E2F3 in ESCC cells. (A) Computational prediction of interaction between miR-140-3p with the E2F3 3′-UTR sequence. (B) Luciferase reporter assays in ESCC cells transfected with E2F3-WT or E2F3-MUT, along with miR-140-3p mimic or control mimic. (C) Western blotting analysis of E2F3 expression in ESCC cells transfected with (or without) miR-140-3p mimic. (D) Western blotting analysis of E2F3 levels in ESCC cells co-transfected with (or without) miR-140-3p, along with (or without) the E2F3 expression vector. (E) The expression of E2F3 in TCGA ESCC tissues and normal tissues. (F) qRT-PCR analysis of E2F3 expression in ESCC tissues and adjacent normal tissues. (G) The positive correlation between circ_0087378 and E2F3 in ESCC tissues was determined by qRT-PCR assays. (H) The negative correlation between miR-140-3p and E2F3 in ESCC tissues was determined by qRT-PCR assay. (I) E2F3 expression in ESCC cell lines and SHEE cells was measured using qRT-PCR assays. ***P < 0.001; ### P < 0.001.