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. Author manuscript; available in PMC: 2021 Mar 3.
Published in final edited form as: Metabolomics. 2019 May 23;15(6):83. doi: 10.1007/s11306-019-1544-z

Fig. 1.

Fig. 1

Workflow for the harvesting, storage, and targeted metabolomics analysis in skin fibroblasts. Cultured skin fibroblasts (n = 3 per group) were collected using four different methods (Methods I - IV) followed by storage at −80°C for 48 hours, two weeks, or four weeks. Targeted metabolomics analysis of the TCA cycle metabolites was carried out using gas chromatography/mass spectrometry (GC/MS). Amino acids and acylcarnitines were profiled using liquid chromatography/mass spectrometry (LC/MS).