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Testing the primer performance. Panel A: PCR product size after pooling of reaction A and B. Expected sizes of amplicons are from 1,500 bp to 2,093 bp before primer trimming. Panel B: MinION reads after quality control, primer, adapter trimming. Panel C: Reads mapped to SARS-CoV-2 reference genome, before amplicon 2 and 16 primer boosting. Panel D: Reads mapped to SARS-CoV-2 reference genome, after amplicon 2 and 16 primer boosting.
