Table 3. Affinities of ATG8 domains for the integrin β₃ peptide.

K_I values for ATG8 domains calculated from displacement experiments using unphosphorylated integrin β₃ peptide and phosphorylated peptides (pThr⁷⁷⁷, pSer⁷⁷⁸, pThr⁷⁷⁹, pTyr⁷⁸⁵, or pThr⁷⁷⁹ pTyr⁷⁸⁵). Indicated error is the error of the mean (SEM); n = 3 replicates. N.B., no or low affinity (K_I > 100 μM); N.M., not measured.

Peptide sequences	KI values ± SEM (μM)
	MAP1LC3A ATG8	MAP1LC3B ATG8	MAP1LC3C ATG8	GABARAPL2 ATG8	GABARAP ATG8
Unphosphorylated CH3CO- KEATSTFTNITYRG-NH2	N.B.	N.B.	N.B.	N.B.	N.B.
pThr777 CH3CO- KEApTSTFTNITYRG-NH2	N.B.	N.B.	73 ± 4	N.M.	47 ± 3
pSer778 CH3CO- KEATpSTFTNITYRG-NH2	29.4 ± 0.5	N.B.	16.1 ± 0.5	80 ± 5	N.M.
pThr779 CH3CO- KEATSpTFTNITYRG-NH2	N.B.	N.B.	82 ± 3	N.B.	N.M.
pTyr785 CH3CO- KEATSTFTNITpYRG-NH2	38 ± 2	81 ± 2	83 ± 4	89 ± 12	N.M.
pThr779/pTyr785 CH3CO- KEATSpTFTNITpYRG-NH2	8.5 ± 0.5	15 ± 1	8 ± 2	N.M.	17.8 ± 0.8