TABLE II.
Non-synonymous Mutations in SARS-CoV-2 Structural Proteins
| Gene | Nucleotide mutation | Amino acid mutation | Biochemical property | 59MDW sample(s) | Frequency |
|---|---|---|---|---|---|
| S | 21,642 G > C | R21T | Positively charged to polar but neutral | 3829 | 2.70% |
| S | 22,245 G > T | D228Y | Negatively charged to polar but neutral | 3680 | 2.70% |
| S | 23,403 A > G | D614G | Negatively charged to nonpolar | All | 100.00% |
| S | 23,625 C > T | A688V | Both nonpolar | 3829, 3831, 3784, 2940, 2913, 3014 | 16.22% |
| S | 2,375 A > G | T732A | Polar but neutral to nonpolar | 3,786 | 2.70% |
| S | 24,236 G > T | A892S | Nonpolar to polar but neutral | 3807, 3723, 3804, 3776, 3808 | 13.51% |
| S | 24,770 G > T | A1070S | Nonpolar to polar but neutral | 3784 | 2.70% |
| E | 26,455 C > T | P71S | Nonpolar to polar but neutral | 2973, 3488, 2956 | 8.11% |
| N | 28,362 C > G | G30A | Both nonpolar | 3654, 3681, 3096, 3709, 3680 | 13.51% |
| N | 28,845 C > T | R191L | Positively charged to nonpolar | 3654, 3681, 3096, 3709, 3680 | 13.51% |
| N | 28,775 C > T | P168S | Nonpolar to polar but neutral | 3488 | 2.70% |
| N | 28,821 C > A | S183Y | Both polar but neutral | 3014, 2913, 3784, 2940, 3829, 3831, 3717, 3535, 3687, 3724, 3540, 3537, 3802, 3542, 3667, 3808, 3776, 3804, 3273, 3807 | 54.05% |
| N | 28,881 G > A | R203K | Both positively charged | 2576, 3786, 3634 | 8.11% |
| N | 28,882 G > A | G204R | Nonpolar to positively charged | 2576, 3786, 3634 | 8.11% |
SARS-CoV-2 whole-genome sequencing revealed 14 non-synonymous mutations in genes encoding structural proteins (S = spike; E = envelope; N = nucleocapsid) in 59MDW specimens. The biochemical properties of each non-synonymous change was characterized using the NCBI structure program, and the mutation frequency was calculated by dividing the number of specimens with a mutation by the total number of sequenced clinical specimens (N = 37).