Fig. 1.

Device design. a The chip consists primarily of a PDMS chamber bonded to a glass slide which has been patterned with a micro heater and temperature sensor. Key dimensions of the PDMS microchamber have been included in projected top and side views (all dimensions in mm). b Also shown is the design of an integrated microfluidic device, in which weirs retain microbeads in the chamber while beads are exposed to streams of buffer or reagents