Fig. 2.

Plasmid-borne rec10 NLS mutations block entry of other LinE proteins into the nucleus. Strains bearing rec25–GFP and a complete rec10 deletion on the chromosome and carrying a plasmid with rec10⁺ (WT), with the indicated rec10 NLS mutation, or without rec10 (rec10Δ) were harvested during asynchronous (h⁹⁰) meiosis and examined by light microscopy. In the merged images, green indicates Rec25–GFP, and red indicates chromatin in the nucleus; yellow is their overlap. The dashed line outlines the cell. ΔA indicates deletion of Rec10 NLS site A, and AlaA indicates substitution of alanine for each of the four amino acids of site A; site B was similarly deleted or mutated, indicated as ΔB and AlaB, respectively. Combinations of these mutations are indicated by ΔA ΔB or AlaA AlaB. Images are representative of >50 cells from three separate cultures analyzed on different days. Cells are in the horsetail stage, during which the nucleus moves repeatedly from one end of the cell to the other; nuclei are longer when in the middle of the cell than when at either end, when the nucleus becomes nearly round (Robinow, 1977; Bähler et al., 1993; Chikashige et al., 1994). Scale bars: 5 μm.