Fig. 3.

Chromosomal rec10 NLS mutations block entry of Rec10–GFP into the nucleus. Strains without (WT) or with a rec10 NLS mutation were harvested during asynchronous (h⁹⁰) meiosis and examined by light microscopy. Green indicates Rec10, and red indicates chromatin in the nucleus; yellow is their overlap. The dashed line outlines the cell. NLS mutations are designated as in Fig. 2. Images are representative of >50 cells from three separate cultures analyzed on different days. Cells are in the horsetail stage, as in Fig. 2. Scale bars: 5 μm.